84-5254-28　NAT10 200ul　525796

特徴

• RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs (PubMed:25411247, PubMed:25653167).
• Catalyzes the formation of N4-acetylcytidine (ac4C) at position 1842 in 18S rRNA (PubMed:25411247).
• May also catalyze the formation of ac4C at position 1337 in 18S rRNA (By similarity).
• Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis (PubMed:25411247, PubMed:25653167).
• Catalyzes the formation of ac4C in serine and leucine tRNAs (By similarity).
• Requires the tRNA-binding adapter protein THUMBD1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation (PubMed:25653167).
• Can acetylate both histones and microtubules (PubMed:14592445, PubMed:17631499, PubMed:19303003).
• Histone acetylation may regulate transcription and mitotic chromosome de-condensation (PubMed:17631499).
• Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization (PubMed:14592445, PubMed:18082603).
• Acetylates alpha-tubulin, which may affect microtubule stability and cell division (PubMed:19303003).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.