Base excision repair (BER) proteins catalyze the removal of incorrect or damaged bases, including oxidized bases, from DNA.
N-glycosylases specific to a given lesion remove the incorrect base as the first step in BER.
MYH is the mammalian ortholog of E.
coli MutY, a DNA glycosylase that catalyzes the removal of 8-oxoG:A mismatches (1).
Several MYH isoforms have been detected in human cells localizing to either the nucleus or the mitochondria (2).
MYH interacts with DNA repair proteins and localizes to DNA damage foci after oxidative damage (3).
Research studies have shown that mutations in the corresponding MYH gene are associated with human gastric (4) and colorectal (5-7) cancers.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human MUTYH. Species Crossreactivity: Mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human MUTYH.
