The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta, part of the post-replicative DNA mismatch repair system.
MutS beta initiates mismatch repair by binding to a mismatch and then forming a complex with MutL alpha heterodimer.
This gene contains a polymorphic 9 bp tandem repeat sequence in the first exon.
The repeat is present 6 times in the reference genome sequence and 3-7 repeats have been reported.
Defects in this gene are a cause of susceptibility to endometrial cancer.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human MSH3. Species Crossreactivity: Rat
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to human MSH3 at N-terminal.
