The Drosophila piwi gene was identified as being required for the self-renewal of germline stem cells (1).
Piwi homologs are well conserved among various species including Arabidopsis, C.
elegans, and Homo sapiens (1).
Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2).
Miwi and Mili bind to Piwi- interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5).
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200 Immunofluorescence: 1:20-1:100 Immunoprecipitation: 1:20-1:100ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human MIWI. Species Crossreactivity: Human, mouse and rat
