84-5244-19　MDK 100ul　525265

特徴

• Midkine, or MK, is a heparin-binding molecule involved in the regulation of growth and differentiation during embryogenesis.
• MK expression is tightly regulated during embryonic development by steroid receptors of the retinoic acid superfamily.
• The mature human MK protein is 118aa in length and contains five intrachain disulfide bonds.
• MK is a non-glycosylated protein that shows greater than 87% identity between human and mouse.
• The C-terminus of MK contains the principle heparin-binding site and the molecule’s neurite-promoting sequences; both the N-terminal and C-terminal sequences are required for the molecule’s neurotrophic properties.
• An association between overexpression of MK and colon adenocarcinoma has been shown in families suffering from familial polyposis.
• In addition, MK functions to enhance the activity of plasminogen activator (PA).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.