84-5244-18　mDia1 200ul　525264

特徴

• Acts in a Rho-dependent manner to recruit PFY1 to the membrane.
• Required for the assembly of F-actin structures, such as actin cables and stress fibers.
• Nucleates actin filaments.
• Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization.
• Required for cytokinesis, and transcriptional activation of the serum response factor.
• DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics.
• Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity).
• Has neurite outgrowth promoting activity (By similarity).
• In hear cells, it may play a role in the regulation of actin polymerization in hair cells.
• The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex.
• It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity.
• In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
• Plays a role in the regulation of cell morphology and cytoskeletal organization.
• Required in the control of cell shape (PubMed:20937854, PubMed:21834987).
• Plays a role in brain development (PubMed:24781755
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.