Acts as a transcriptional activator or repressor (PubMed:27181683).
Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells.
Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation.
Involved in renal tubule survival and F4/80 maturation.
Activates the insulin and glucagon promoters.
Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element.
SUMO modification controls its transcriptional activity and ability to specify macrophage fate.
Binds element G1 on the glucagon promoter (By similarity).
Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human MafB. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human MafB at C-terminal. Species sequence homology: porcine (100%), zebrafish (83%), bovine (100%), equine (100%), rabbit (92%), canine (100%), chicken (83%), xenopus (83%)
