May play a role in the phosphorylation of proteins central to Parkinson disease.
Phosphorylates PRDX3.
May also have GTPase activity.
Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway.
The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increasein lysosomal pH, and calcium release from lysosomes.
Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary Ab in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human LRRK2. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human LRRK2 at C-terminal. Species sequence homology: porcine (100%), zebrafish (88%), bovine (100%), equine (88%), sheep (100%), rabbit (100%), canine (100%), chicken (100%), xenopus (88%)
