84-5238-45　LIMK1 100ul　524952

特徴

• There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain.
• LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers.
• Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions.
• LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain.
• LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development.
• LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.
• Two splice variant have been identified.
• Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.