84-5236-10　KRT17 100ul　524829

特徴

• Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells.
• Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2).
• Keratin isoforms demonstrate tissue- and differentiation specific profiles that make them useful as biomarkers (1).
• Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
• Keratin 17 is involved in wound healing and cell growth, two processes that require rapid cytoskeletal remodeling (7).
• Keratinocytes deficient in keratin 17 exhibit abnormal Akt/mTOR signaling and fail to produce an increase in translation, cell size, or growth; these cells also exhibit abnormal 14-3-3 sigma localization.
• As 14-3-3 sigma typically associates with keratin 17, these results imply that Akt/mTOR signaling results in sequestration of 14-3-3 sigma with keratin 17 in the cytosol, which is required for translation and cell growth.
• Phosphorylation of keratin 17 on Ser44 may provide a docking site for 14-3-3 sigma binding (8).
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.