84-5235-87　KPNA2 100ul　524816

特徴

• The import of proteins into the nucleus is a process that involves at least 2 steps.
• The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex.
• Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic aa’s or 2 such regions spaced about 10aa apart.
• Proteins involved in the first step of nuclear import have been identified in different systems.
• These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature- sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS.
• KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins.
• KPNA2 also may play a role in V(D)J recombination
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.