84-5235-64　KLKB1 200ul　524804

特徴

• Plasma prekallikrein is a glycoprotein that participates in the surface dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation.
• It is synthesized in the liver and secreted into the blood as a single polypeptide chain.
• Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond.
• Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond.
• The heavy chain originates from the N-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91aa.
• Each repeat harbors a novel structure called the apple domain.
• The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein.
• The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases.
• Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.