84-5235-37　KLF4, phosphorylated (Ser254) 100ul　524784

特徴

• Transcription factor; can act both as activator and as repressor.
• Binds the 5'-CACCC-3' core sequence.
• Binds to the promoter region of its own gene and can activate its own transcription.
• Regulates the expression of key transcription factors during embryonic development.
• Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation.
• Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface.
• Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.
• Contributes to the down-regulation of p53/TP53 transcription.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Peptide ELISA, Immunofluorescence and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200.
• Formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer is recommended
• Peptide ELISA: 1ug/mlImmunofluorescence (IC): 1;100-1;500Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.