84-5233-29　KCNMB1 100ul　524672

特徴

• Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel.
• Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity.
• Increases the apparent Ca2+/voltage sensitivity of the KCNMA1 channel.
• It also modifies KCNMA1 channel kinetics and alters its pharmacological properties.
• It slows down the activation and the deactivation kinetics of the channel.
• Acts as a negative regulator of smooth muscle contraction by enhancing the calcium sensitivity to KCNMA1.
• Its presence is also a requirement for internal binding of the KCNMA1 channel opener dehydrosoyasaponin I (DHS-1) triterpene glycoside and for external binding of the agonist hormone 17-beta-estradiol (E2).
• Increases the binding activity of charybdotoxin (CTX) toxin to KCNMA1 peptide blocker by increasing the CTX association rate and decreasing the dissociation rate.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight.
• Immunohistochemistry: 1:50-1:200, Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.