84-5231-59　JIP1, phosphorylated (Thr103) 100ul　524586

特徴

• The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module.
• Required for JNK activation in response to excitotoxic stress.
• Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and inhibiting JNK phosphorylation of c-Jun.
• May also participate in ApoER2-specific reelin signaling.
• Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function.
• Appears to have a role in cell signaling in mature and developing nerve terminals.
• May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity).
• Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response.
• Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.