84-5227-82　IL7 200ul　524388

特徴

• IL-7 plays a key role in lymphopoiesis and lymphoid homeostasis (1).
• Stromal and epithelial cells within the bone marrow and thymus produce IL-7 (1).
• The primary targets of IL-7 are T cells, B cells, and dendritic cells (1).
• IL-7 is crucial for T cell development, the importance of which is underscored by the lack of T cells in both mice and humans that are deficient in IL-7/IL-7R signaling (1,2).
• While IL-7 appears to be required for B cell development in mice, the role of IL-7 in human B cell development is unclear (1,3).
• In addition to its effects on T cell lymphopoiesis, IL-7 promotes the maintenance and survival of na ve and memory alpha beta T cells, as well as gamma delta T cells (1).
• The IL-7 receptor is a heterodimer of the common gamma chain, gamma c, and the IL-7-specific IL-7R alpha (1).
• IL-7 activates PI3K/Akt, Jak1/2, and Stat1, 3, and 5 (1).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.