Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils.
Primes CD4+ T- cells for IL-2 and IL-15 responsiveness.
Also induces T- lymphocyte expression of interleukin 2 receptor.
Ligand for CD4.;Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.;Isoform 3 is involved in cell cycle progression in T-cells.
Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene.
May act as a scaffold for GABPB1 (the DNA- binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight.
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total IL16. Species Crossreactivity: Human and mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to IL16 at C-terminal. Species sequence homology: Zebrafish (83%), Bovine (100%), Equine (100%), Sheep (100%), Rabbit (83%), Canine (92%)
