The genes encoding type I interferons (IFNs), which include 14 IFN- genes, one IFN-∫ gene, one IFN-w (also known as IFN- II1) gene, and a number of IFN-w pseudogenes, are clustered on human chromosome 9.
Interferons- and -∫ are cytokines that are widely known to induce potent antiviral activity.
IFN- and -∫ exert a variety of other biological effects, including antitumor and immunomodulatory activities and are increasingly used clinically to treat a range of malignancies, myelodysplasias and autoimmune diseases.
IFN-w is antigenically different from human IFN- , IFN-∫ or IFN- , but is a component of natural mixtures of IFN species produced by virus-induced leukocytes or Burkitt's lymphoma cells.
The type I interferon receptor (IFN- R) interacts with IFN- , IFN-∫ and IFN-w, and seems to be a multisubunit receptor.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human IFNB1. Species Crossreactivity: Mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human IFNB1.
