The cDNA encodes a 282aa predicted protein containing a leucine zipper motif at the amino terminus.
Unlike most other leucine zipper proteins, IFP35 lacks a basic domain associated with DNA binding.
Northern blots showed that the 1.4-kb IFP35 mRNA occurs in fibroblasts, macrophages, and epithelial cells following interferon treatment.
Western blots indicated that the IFP35 gene product can form homodimers and that there was increased nuclear localization following interferon treatment.
Among the exons identified from a phage P1 artificial chromosome (PAC) that included regions from BRCA1 were several coding regions with 100% identity to IFP35.
IFP35 gene situated centromeric to the BRCA1 gene in the course of sequencing and analyzing 117 kb of DNA from the 17q21 region.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total IN35. Species Crossreactivity: Human and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to IN35 at C-terminal.
