84-5223-95　IDH1 100ul　524184

特徴

• IDH1 is one of three isocitrate dehydrogenases that catalyze the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (alpha-KG).
• These enzymes exist in two distinct subclasses that utilize either NAD or NADP+ respectively, as an electron acceptor (1).
• IDH1 is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes.
• IDH2 and 3 are mitochodrial enzymes that also function in the Krebs cycle.
• IDH1 is inactivated by phosphorylation at Ser113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock (2,3).
• IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle.
• Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma (4).
• IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway (5).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.