84-5223-89　ID2 100ul　524181

特徴

• Inhibitor of DNA-binding-2 (Id2) is a member of the Id proteins which belong to the helix-loop-helix (HLH) protein family.
• The Id protein functions by binding to specific transcription factors and preventing their dimerization and DNA binding (1-3).
• Id2 interacts with a wide variety of transcription factors including E proteins (5), TCS (4), Pax (6) and the tumor suppressor Rb (1).
• Id2 has been shown to be important in regulating cellular differentiation, proliferation, development and tumorgenesis (7-9).
• In tumor cells, increased levels of Id2 functionally inactivate Rb, leading to cellular transformation and cancer (10,11).
• Id2 is therefore a promising therapeutic target for treatment of cancer (12).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.