Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans.
Participates in extracellular matrix (ECM) degradation and remodeling.
Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group.
Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety.
It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes.
Facilitates cell migration associated with metastasis, wound healing and inflammation.
Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas.
Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII.
Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity.
Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion.
Heparin increases this AKT1/PKB activation.
Regulates osteogenesis.
Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF.
Implicated in hair follicle inner root sheath differentiation and hair homeostasis.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human HPSE. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human HPSE. Species sequence homology: Porcine (92%), Zebrafish (100%), Bovine (83%), Equine (100%), Sheep (92%), Rabbit (100%), Canine (100%), Chicken (83%)
