Promotes transcription activation in differentiated myotubes (By similarity).
Binds to double- and single-stranded DNA sequences.
Binds to the transcription suppressor CATR sequence of the COX5B promoter (By similarity).
Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs.
Binds both to nuclear and cytoplasmic poly(A) mRNAs.
Binds to poly(G) and poly(A), but not to poly(U) or poly(C) RNA homopolymers.
Binds to the 5'-ACUAGC-3' RNA consensus sequence.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of total human HNRPDL. Species Crossreactivity: mouse and rat
Isotype:IgG
Calc Applications Abbrev:E IC IF IHC WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Synthetic peptide corresponding to human HNRPDL at C-terminal. Species sequence homology: porcine, bovine, equine, sheep, rabbit, chicken and xenopus