Multifunctional protein with various roles in different cellular compartments.
May act in a redox sensitive manner.
Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA.
Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity).
Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (By similarity).
Negatively regulates B-cell and myeloid cell differentiation.
In hematopoietic stem cells may regulate the balance between self-renewal and differentiation.
Involved in negative regulation of canonical Wnt signaling (By similarity).
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human HMGB3. Species Crossreactivity: Mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human HMGB3. Species sequence homology: porcine(100%), bovine(100%), equine(100%), sheep (100%), rabbit(100%), canine(100%), chicken(100%), xenopus (100%)
