Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.
Nucleosomes consist of approximately 146bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4).
The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
This gene is intronless and encodes a member of the histone H4 family.
Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry, ELISA, Immunoprecipitation and CHIP.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunofluorescence: 1:50-1:200Immunoprecipitation: 1:50-1:100ChIP: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total Histone H4R3me2a. Species Crossreactivity: Mouse and Rat
Isotype：IgG
Calc Applications Abbrev：ChIP E IC IF IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4.
