Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes.
The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1).
The N-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5).
These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6).
In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7).
Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56.
Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3).
Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10).
Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin.
Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry, ELISA, Immunoprecipitation, CHIP.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Immunoprecipitation: 1:50-1:200ChIP: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total Histone H3K9me1. Species Crossreactivity: Human and Mouse
Isotype：IgG
Calc Applications Abbrev：ChIP E IC IF IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3.
