84-5217-41　HIPK1/2/3 200ul　523846

特徴

• Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis.
• Plays a role as a corepressor for homeodomain transcription factors.
• Phosphorylates DAXX and MYB.
• Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm.
• Inactivates MYB transcription factor activity by phosphorylation.
• Prevents MAP3K5-JNK activation in the absence of TNF.
• TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis.
• May be involved in anti-oxidative stress responses.
• Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis.
• Promotes angiogenesis and to be involved in erythroid differentiation.
• May be involved in malignant squamous cell tumor formation.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.