HDAC9 is a transcriptional regulator of the histone deacetylase family, subfamily 2.
Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4.
Plays an important role in transcriptional regulation, cell cycle progression and developmental events.
Histone deacetylases act via the formation of large multiprotein complexes.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:3000, incubate membrane with diluted Ab in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography from serum.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of total human HDAC9.
Isotype:IgG
Calc Applications Abbrev:E IC IF IHC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to human HDAC9.