84-5213-07　GRB10, phosphorylated (Ser476) 100ul　523612

特徴

• Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways.
• Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors.
• The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation.
• Delays and reduces AKT1 phosphorylation in response to insulin stimulation.
• Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation.
• Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways.
• May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation.
• Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6.
• Positive regulator of the KDR/VEGFR-2 signaling pathway.
• May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.