Receptor for metastin (kisspeptin-54 or kp-54), a C-terminal amidated peptide of KiSS1.
KiSS1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some breast carcinomas without affecting tumorigenicity.
The metastasis suppressor properties maybe mediated in part by cell cycle arrest and induction of apoptosis in malignant cells.
The receptor is essential for normal gonadotropin-released hormone physiology and for puberty.
The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis atpuberty and in adulthood.
The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself.
Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calciumrelease through pertussis toxin-insensitive G(q) proteins.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary Ab in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human GPR54. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human GPR54 at C-terminal.
