84-5208-41　GNA15 200ul　523380

特徴

• Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1).
• Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenylyl cyclase), which act to generate one or more intracellular messengers, are less numerous.
• In mammals, G protein , ∫ and polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5).
• Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors.
• Four distinct classes of G subunits have been identified; these include Gs, Gi, Gq and Ga 12/13 (3,4).
• The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Ga i-1, Ga i-2, Ga i-3, Ga o, Ga t1, Ga t2, Ga z and Ga gust (4).
• Of these, the three Ga i subtypes function to open atrial potassium channels (6).
• Ga 16 is a member of the Gq subfamily and is expressed specifically in hematopoietic cells (7).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.