Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of alpha, beta and gamma subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli.
To date, over 20 known G alpha subunits have been classified into four families, G alpha(s), G alpha(i/o), G alpha(q) and G alpha(12), based on structural and functional similarities (1,2).
Phosphorylation of Tyr356 of G alpha(q)/G alpha(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of G alpha with receptors and abolishes ligand-induced IP3 formation (3).
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human GNA11. Species Crossreactivity: Mouse, rat and porcine
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to human GNA11. Species sequence homology: Bovine (100%), Equine (100%), Sheep (100%), Canine (100%), Chicken (100%), Xenopus (100%)
