84-5207-77　Glucocorticoid Receptor, phosphorylated (Ser203) 100ul　523348

特徴

• The protein encoded by this gene is a receptor for glucocorticoids and can act as both a transcription factor and a regulator of other transcription factors.
• The encoded protein can bind DNA as a homodimer or as a heterodimer with another protein such as the retinoid X receptor.
• This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins.
• The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus.
• Mutations in this gene are a cause of glucocorticoid resistance, or cortisol resistance.
• Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined.
• Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.