GANP is a member of the SAC3 family of nuclear export factors.
In combination with ENY2, PCID2 and centrins, forms the TREX-2 complex that links transcription/ processing with nuclear mRNA export.
GANP, required to recruit ENY2 to nuclear pore complexes (NPCs), requires both its CID and MCM3AP domains, together with nucleoporin Nup153, for recruitment to NPCs.
The MCM3AP domain, corresponding to the carboxy-terminal 721aa of GANP, shows homology with the Gcn5-related N acetyltransferase superfamily.
The protein MCM3AP is encoded entirely within the 3? region of the GANP gene.
MCM3AP transcription begins within intron 16 of GANP immediately upstream of the first MCM3AP exon, where NF-IL6, AP-1, NF-?B, and p53 consensus transcription factor binding sites are located.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:3000Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human GANP. Species Crossreactivity: Mouse
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human GANP.
