84-5203-92 G3BP-1, phosphorylated (Ser232) 100ul 523144
特徴
- G3BP-1 an hnRNA-binding protein and endoribonuclease that participates in the Ras signal transduction pathway.
- A regulated effector of stress granule (SG) assembly.
- SGs are involved in mRNA sorting in the storing of untranslated mRNAs.
- Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
- Other applications not tested.
- Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
- Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
- Storage and Stability:May be stored at 4°C for short-term only.
- Aliquot to avoid repeated freezing and thawing.
- Store at -20°C.
- Aliquots are stable for 12 months after receipt.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
- Size：100ul
- Host：rabbit
- Source Antibody：human
- Grade：Affinity Purified
- Purity：Purified by immunoaffinity chromatography from serum.
- Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Specificity：Recognizes endogenous levels of human Phospho-G3BP-1 (Ser232). Species Crossreactivity: Mouse
- Isotype：IgG
- Calc Applications Abbrev：E IHC WB
- Calc Crossreactivity：Hu Mo
- Immunogen：Synthetic peptide corresponding to human G3BP-1 around the phosphorylation site of Serine 232.
- Swiss Prot Number：Q13283
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