Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor.
In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites.
On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling.
Has a critical function in regulating the development of cells destined to form and maintain the skeleton.
It is thought to have an important role in signal transduction, cell proliferation and differentiation.
In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A.
This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Phospho-FOS (Ser32). Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthesized peptide derived from human FOS around the phosphorylation site of Ser32. Species sequence homology: Porcine (100%), Bovine (100%), Equine (100%), Rabbit (100%), Canine (100%), Chicken (83%)
