Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry, Immunoprecipitation, ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight.0Immunofluorescence (IF-IC): 1:200Immunoprecipitation: 1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes C-terminal, internal, and N-terminal Flag-tag fusion proteins. Species Crossreactivity: All
Isotype：IgG
Calc Applications Abbrev：E IC IF IP WB
Immunogen：A synthetic peptide DYKDDDDK coupled to KLH.
