This gene encodes coagulation factor III which is a cell surface glycoprotein.
This factor enables cells to initiate the blood coagulation cascades, and it functions as the high- affinity receptor for the coagulation factor VII.
The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis.
Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces.
There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic.
This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described.
Alternate splicing results in multiple transcript variants.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human F3. Species Crossreactivity: Mouse
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human F3.
