ERK1 p42 MAP kinase plays a critical role in the regulation of cell growth and differentiation.
Activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters.
ERK2 p44 MAP kinase plays a critical role in the regulation of cell growth and differentiation.
Acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:100-1:500Immunofluorescence: 1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human ERK1/2. Species Crossreactivity: mouse, rat, porcine, zebrafish, bovine, equine, sheep, canine, monkey, fish
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Bo Ca Eq Hu Mk Mo Po Rt Sh Ze
Immunogen：Synthetic peptide corresponding to human ERK1/2.
