84-5196-67　ERCC2 200ul　522749

特徴

• ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.
• In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment.
• The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening.
• In transcription, TFIIH has an essential role in transcription initiation.
• When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape.
• Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
• XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex.
• Involved in the regulation of vitamin-D receptor activity.
• As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation.
• Might have a role in aging process and could play a causative role in the generation of skin cancers.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.