84-5196-41 EPS15, phosphorylated (Tyr849) 200ul 522732
特徴
- This gene encodes a protein that is part of the EGFR pathway.
- The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF.
- Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias.
- Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
- Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA.
- Other applications not tested.
- Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
- Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
- Storage and Stability:May be stored at 4°C for short-term only.
- Aliquot to avoid repeated freezing and thawing.
- Store at -20°C.
- Aliquots are stable for 12 months after receipt.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
- Size：200ul
- Host：rabbit
- Source Antibody：human
- Grade：Affinity Purified
- Purity：Purified by affinity chromatography from serum.
- Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Specificity：Recognizes endogenous levels of human EPS15 only when phosphorylated at Tyrosine 849. Species Crossreactivity: mouse and rat
- Isotype：IgG
- Calc Applications Abbrev：E IC IF WB
- Calc Crossreactivity：Hu Mo Rt
- Immunogen：Synthetic peptide corresponding to human EPS15 around the phosphorylation site of Tyrosine 849.
- Swiss Prot Number：P42566
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