Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development.
Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells.
The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.
May play a pivotal role in forebrain function.
Binds to, and induce the collapse of, commissural axons/growth cones in vitro.
May play a role in constraining the orientation of longitudinally projecting axons (By similarity).
Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human Ephrin-B3.
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human Ephrin-B3. Species sequence homology: porcine (100%), bovine (100%), equine (100%), sheep 100%), rabbit (100%), canine (100%)
