Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 (E-NPP 7) also known as alkaline sphingomyelin phosphodiesterase (Alk-SMase) or intestinal alkaline sphingomyelinase is an enzyme that in humans is encoded by the ENPP7 gene.
Converts sphingomyelin to ceramide.
Also has phospholipase C activity toward palmitoyl lyso- phosphocholine.
Does not appear to have nucleotide pyrophosphatase activity.
Inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion.
Not inhibited by ADP, AMP and EDTA.
Detected in the colon (at protein level).
Expressed in the duodenum, jejunum and liver and at low levels in the ileum.
Expression was very low in the esophagus, stomach and colon.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total ENPP7. Species Crossreactivity: Mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human ENPP7. Species sequence homology: Porcine (92%), Bovine (100%), Equine (92%), Sheep (100%), Rabbit (100%), Canine (92%), Chicken (92%), Xenopus (83%)
