Emerin is a broadly expressed integral protein of the nuclear inner membrane (1).
It contains a LEM domain and binds to several nuclear proteins, such as BAF (barrier-to- autointegration factor) and A- and B-type lamins, which are important in nuclear functions (2-5).
Emerin may regulate gene expression through binding to other transcriptional regulators (6,7).
Emerin binds to Beta-catenin and inhibits its nuclear accumulation (8).
Recent studies demonstrate that Emerin is required for HIV-1 infectivity (9).
Mutations in Emerin are a major cause of Emery-Dreifuss muscular dystrophy (EDMD), a disorder characterized by progressive skeletal muscle weakening (10).
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human EMD. Species Crossreactivity: Mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human EMD. Species sequence homology: Porcine (100%), Bovine (88%), Equine (100%), Rabbit (100%), Canine (100%)
