84-5194-16　EIF5A 100ul　522605

特徴

• mRNA-binding protein involved in translation elongation.
• Has an important function at the level of Mrna turnover, probably acting downstream of decapping.
• Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity.
• With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis.
• Regulates also TNF-alpha-mediated apoptosis.
• Mediates effects of polyamines on neuronal process extension and survival.
• May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.
• Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.