84-5192-21　EEF1D 200ul　522501

特徴

• This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome.
• This subunit, delta, functions as guanine nucleotide exchange factor.
• It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat.
• This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins.
• Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene.
• Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.