Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities.
Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins.
Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.
Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary Ab in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human EDG2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human EDG2 at N-terminal. Species sequence homology: porcine (%), bovine (%), equine (%), sheep (%), rabbit (%), canine (%), chicken (%)
