84-5191-06　E2F1 200ul　522444

特徴

• The E2F transcription factors are essential for regulation of the cell cycle (1,2).
• Physiological E2F is a heterodimer composed of an E2F subunit together with a DP subunit (3,4).
• Six members of the E2F family have been identified, and each E2F subunit has a DNA binding and a dimerization domain.
• E2F-1 to -5 activate transcription.
• E2F-1 to -3 bind pRb, and E2F-4 and -5 bind p107 or p130, and these interactions are under cell cycle control (5-8).
• E2F-1 has oncogenic properties in vivo and in vitro.
• E2F-1 can induce apoptosis through p53-dependent and -independent mechanisms.
• E2F-1 is stress-responsive, and is regulated by a PI3-kinase-like kinase family such as the ATM/ATR kinases (9-11).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.