84-5189-18　DOK3 200ul　522348

特徴

• Docking proteins interact with receptor tyrosine kinases and mediate particular biological responses.
• DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP.
• May modulate Abl function.
• On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP via their SH2 domains.
• Both Tyr-381 and Tyr-398 are required for interaction with INPP5D.
• Only Tyr-381 is required for interaction with CSK.
• Binds ABL through the PTB domain and in a kinase-dependent manner.
• Does not interact with RasGAP.
• 4 isoforms of the human protein are produced by alternative splicing.
• Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.