Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and development (1,2).
Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3 (1,2).
DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication.
DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues.
DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA.
DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation.DNMT3L is a catalytically inactive regulatory factor for the DNMT3A and DNMT3B de novo methyltransferases that is expressed at low levels in embryonic stem cells, testis, ovaries, and thymus (1,2).
These de novo methyltransferases consist of a heterotetrameric complex containing two molecules of DNMT3L, and either two molecules of DNMT3A or DNMT3B (3).
DNMT3L contains an N-terminal ATRX- DNMT3-DNMT3L (ADD) domain and a C-terminal methyltransferase-like domain (4-7).
The methyltransferase- like domain binds to DNMT3A and DNMT3B to stimulate catalytic activity by increasing the binding of S- adenosylmethionine and DNA (4,5).
The ADD domain recruits the methyltransferase complex to transcriptionally inactive regions of the genome by binding to unmethylated histone H3 Lys4 (6,7).
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry, ELISA, Immunoprecipitation, CHIP.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:20-1:50Immunoprecipitation: 1:20-1:50ChIP: 1:20-1:50ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human DNMT3L. Species Crossreactivity: Mouse
Isotype：IgG
Calc Applications Abbrev：ChIP E IC IF IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human DNMT3L. Species sequence homology: Porcine (90%), Canine (89%)
